Is there specific programmable freezer equipment for cryopreservation?

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Does the dark J-V curve of bifacial thin film devices illuminated through both sides should always converge?

In my case, thicker devices (> 2μm ) sometimes do not converge when I illuminated from the front and Illuminated through the back. However, for thin devices, the dark J-V curve always...

14 April 2020 6,765 0 View

What should be the composition and weight % of standard calibration gas mixture for the analysis of CO2 reduction products ?

I have a GC with dual packed column (Hayesep-A and Molsieve-13x). This GC will be used to analyze the product gases generated from the reactor by photochemical and electrochemical CO2 reduction....

20 September 2017 6,910 1 View

Modelling the impact into soil in Abaqus

Hi everyone, I am trying to model the impact of a spear into the soil in Abaqus. I modelled it as an axisymmetric model and used Drucker-Prager plasticity model for the soil. Every time I run...

14 March 2017 1,099 7 View

I would like to analysis the fatty acid profile in Spirogyra filamentous algae sample. Kindly suggest me a suitable protocol for FAME analysis?

I do not know how to prepare my sample (Spirogyra species) for FAME analysis. So please suggest me a suitable protocol for FAME analysis for my sample.

08 November 2016 2,077 1 View

Could you please suggest me which database in Canada has information on anxiety during pregnancy?

I am working on anxiety during pregnancy and neighbourhood socioeconomic status for preterm birth.

05 January 2016 3,215 2 View

How can I corroborate the OCP measurement with salt spray test?

Phosphated carbon steel showed a better corrosion resistance than bare carbon steel but OCP measurement shows the reverse result.The OCP value for bare carbon steel is -520 mV and that of...

06 February 2014 4,722 3 View

How to calculate faradaic efficiency for hydrogen evolution in solar water splitting?

I am working on photoelectrochemical water splitting using CdS-NaNbO3 as a photo-anode. I have both the photocatalytic hydrogen generation data for the powder photocatalyst and the photocurrent...

20 January 2014 6,425 6 View

Hard to detect GAPDH and Beta actin in exosomes

I isolated exosomes form human serum using exosome isolation kit. I lysed the exosome pellet in 1X RIPA buffer with protease inhibitor. I also cultured the fibroblast cells and used the 1XRIPA...

01 January 1970 9,297 5 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

My article: The Extended Pedersen hypothesis can you correct the the name of first Author?

In My article: The Extended Pedersen hypothesis (published, February 1988 Clinical Physiology and Biochemistry 6(2):68-73,) there is mistake of the name of first Author: it is not Catherine...

11 July 2024 1,801 2 View

Is it possible to generate a (semi-)permanent "transcription bubble" within a replicable plasmid?

I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...

07 July 2024 8,275 5 View

How to dilute essential oil from plants to assess the biological activity?

I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...

04 July 2024 8,100 4 View

Nutrition map: What does it mean for you?

Unluckily, just similar to an expensive car, your brain can be damaged if you ingest anything other than premium fuel.

23 June 2024 2,287 7 View

Have pathomorphological studies of the lungs been performed in patients with desminopathy?

It is known that patients with desminopathy often die from pneumonia. Have pathomorphological studies of the lungs been performed in patients with desminopathy?

15 June 2024 1,355 7 View

Luciferase assay lysis?

Hi! I'm doing a renilla luciferase assay with coelenterazine and HEK293T cells transfected with AP-1 renilla luciferase. I'm wondering if the cells need to be lysed prior to adding coelenterazine...

13 June 2024 9,936 5 View

How to prepare 0.28 U/ml working solution of Acetylcholinesterase inhibition assay for anti-Alzheimer activity?

I have purchased Acetylcholiesterase from Electrophus electricus (electric eel), C3389-2KU, following details are written on the enzyme vial. Type-VI S lyophilized powder 200-1000 Units/mg...

31 May 2024 6,444 1 View

What is the difference between absorption and adsorption?

30 May 2024 4,447 27 View

What effect may biochar types produced from grape stems, leaves, onion leaves and stems, asparragus and cantaloupe stems/leaves have on a harvest?

I'm interested in knowing the differences in the effects caused by four different types of biochar: cantaloupe leaves/stems, onion leaves/stems, grape peels/stems/leaves and asparragus...

28 May 2024 4,907 0 View