How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

What is the difference between actin and loading control in normalization of western blot???

Regarding normalization of western blot analysis, I have found different strategies people follow but I couldn't understand the principle and which one is the accurate, 1- some refer to loading...

04 December 2020 5,721 8 View

Detecting bands by ImageJ on mac not fixed properly in each lane???

I am using ImageJ on mac and when I select bands, the first one detected correctly, when I click cmd 2 (= ctr 2 on windows system) to detect the second band, the rectangle gets fixed on the...

01 December 2020 9,538 4 View

How can i increase the power of the ultrasonic pizeoelcetric sensor to atomize a viscous liquid?

I use a piezoelectric sensor, a humidifier electronic circuit and a 32-volt power supply to generate high-frequency ultrasound waves. I use these waves to atomize the liquid. the problem is that...

16 October 2020 5,097 8 View

How can I solve this error in fluent?

Hello guys. I have one question about UDF in fluent(v202r1). I got a code for my project from one person who wrote it in version ANSYS version 15, when I run it in fluent I have not any problem in...

22 September 2020 3,798 2 View

Best rat model of Type 2 diabetes and metabolic syndrome?

Hi Im seeking advice on what would be the best model of T2D and metabolic syndrome in rats Thank you

04 September 2020 3,661 4 View

Can you recommend a book like "the Classical Field Theories" written by Truesdell and Toupin, in a modern style?

In the literature of continuum mechanics, this book sometimes is called "the Monster". some others call it (and the Non-classical field theories by Treusdell and Noll) "the bibles of continuum...

29 July 2020 5,902 3 View

How can I reconfigure Thermofisher NGS Ion S5 System?

Hi We've got an Ion S5 System (Thermofisher NGS System) at our lab, but suddenly we lost the SSD's (for electrical problems) and now we need to re-install the ubutnu (server and client) and all...

09 June 2020 2,610 1 View

What's the optimal temp. for hot start Taq polymerase? and do we need an special PCR condotion or not?

I've ran a PCR using Hot start Taq polymerase instead of normal taq polymerase by mistake, but as the result i have found some pure and non-sharp bands, may any one help me to find why it has...

04 December 2019 4,010 3 View

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15 January 2019 6,351 3 View

Is there a way to grow biofilm in lab to mimic biofilms in showers and baths? Also, how can I measure it?

Hello, I am new to biofilm research and have a question about growing and measuring biofilms. We want to research the effect of a type of enzymes in breaking down biofilms in showers, baths, hot...

14 February 2021 3,522 3 View

Any idea about the replacement of SmartPak DQ3 Purification Pack, Millipore?

Our Millipore Direct-Q 3 machine is broken down. The water leaks without holding in the purification pack. The Purification Pack was never replaced since its installation in 2013. Is the...

09 November 2020 3,463 4 View

What is the best equipment to find the thermal conductivity of a heatsink?

Dear All, I am trying to find the cooling efficiency of my heatsink and I am wondering if there is any equipment to do this thermal test for me. I am looking to find equipment to find a point to...

30 August 2020 3,961 8 View

Need weather and metrological data for forecasting or predicting the agriculture in Saudi Arabia?

Need weather and metrological data for forecasting or predicting agriculture in Saudi Arabia? This is for the purpose of one of the project by my fellow researchers. Kindly share the same.

11 August 2020 8,163 4 View

I am trying to simulate the Hot rolling process in comsol. Is there anyone who has already done this on comsol? I am facing quite a lot of issues?

I am trying to simulate a Hot rolling process in Comsol. I am facing quite a lot of issues regarding the domain boundary condition and all. If someone who has solved or has an idea of how to...

12 July 2020 1,448 2 View

What is best route for break downing of as-cast CP Titanium?

I can not find literature with comprehensive description about break downing of as-cast of CP Titanium after Vacuum Arc Remelting (VAR) process. what is best rout for hot rolling of CP titanium...

11 July 2020 9,398 2 View

Why do you not include geothermal science as a Research Area?

You provide a list of research topics including Geology, Geochemistry and Geophysics. However, many workers in the geothermal field specialise in the integration of the information from the above...

08 June 2020 9,616 4 View

How can I reduce thermal inertia effect on remote sensing surface temperature images by using closed water bodies?

Dear colleagues, I am searching for clear procedures to follow in reducing thermal inertia effect on remote sensing surface temperature images by using closed water bodies, I need your help...

03 June 2020 6,332 3 View

Modelling heat transfer in soils?

I was wondering if you could help me with a simulation as I am new to the programme, I am new to Abaqus and any help would be appreciated. I need to model heat transfer between the soil of...

10 April 2020 9,583 1 View

Is OPEC+ truly a Cartel or a Tale of Two Countries?

Background Information The knowledge of Economics is, to me, a derived demand - not an end in itself but a means to an end. What do I mean by this? We were thought in school how a cartel operates...

09 April 2020 8,568 9 View