I'm doing an interaction test between erythrocytes of mice and free merozoites of Plasmodium chabaudi. Is there any way to quantify merozoites other than by flow cytometry? I have been trying in the Neubauer chamber, until now without success. I also tried cytospin technique and giemsa, however the counting became a challenge because merozoite measured about 1μm. I thought about immunofluorescence but I'm having a hard time getting a primary antibody.
Any advice would be much appreciated.
Thanks.
Mahmoud Singer
you can accurately count merbozoites by flow cytometry, Flow cytometry is the highest accuracy technique.
but you have to use flow count beads in flow cytometer.
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