It depends if you want the animal to be alive afterwards. If not, you can cut the skin on the backside skull area around the cisterna magna and then, after moving aside the muscles fibers you can get into the cisterna using a glass capillary. The CSF will get into the capillary due to the difference in pressure.

oh wow. can you please share any video or protocol. I do not require mice live after this.

Thanks a lot

Hi, you can look at this video and protocol on JOVE:

https://www.ncbi.nlm.nih.gov/pubmed/19066529

also on RG: Article A Technique for Serial Collection of Cerebrospinal Fluid fro...

Thank you so much Matthias.

Is this protocol working fine? Do you think the brain can be still used for slicing afterwards? Thank you a lot!

Following article might help

Cerebrospinal fluid collection in laboratory mice: Literature review and modified cisternal puncture method

Article Cerebrospinal fluid collection in laboratory mice: Literatur...