Anna Salamero Boix

3 Questions 26 Answers 0 Followers

Questions related from Anna Salamero Boix

Dox treatment of cell lines to induce expression of the sgRNAs?

Dear all, I've a mouse epithelial cell line that is constituvely expressing Cas9, and I infected it with a vector that expresses Dox-inducible gRNA. Cells were selected for Cas9 expression, and...

03 March 2018 1,765 5 View

How do you maintain mouse microglial EOC2? With penicillin-streptomycin?

Dear all, I have mouse microglia EOC2 (CRL-2467) cell line in culture but I think they are growing quite slow. I split them once a week or every 10 days. We maintain them with DMEM, 10% FBS, 2%...

03 March 2017 7,875 2 View

Does anyone know an alternative for 96-well-plates?

Dear all, I would like to know if any of you have some idea about smaller 96-well-plates. Let me explain please. I need to irradiate cells that are seeded in wells as small as the ones that are in...

01 January 2017 9,543 4 View