Hi all, I am currently attempting to generate any one or two particular type of solvents such as ethanol, methanol, etc., for the generation process I wanna use enriched anaerobic fermentation bacteria (Mixed culture) with suggestible carbon source. From the literature I could find only studies operating with pure culture, so kindly suggest me some enrichment process for mixed cultures to convert carbohydrates or VFA's to specific type of solvents.
Acetate/butyrate and carbohydrates can be transformed into ethanol/butanol by Clostridium strains (ABE fermenattion), e.g. with mixed cultures as well. You can find and doiwnload a review (book chapter) about ABE fermentation on my RG page. IF I remember well, an USA group used mixed bacteria strains to increase the yield of alcohols, but the same bacteria can metabolize all three kind of substrate in ABE fermentation.
Look at the work of Chiam Weitzman who developed the technology for converting the molasses into a mixture of acetone, butanol and ethanol. Here is a starting link... http://en.wikipedia.org/wiki/Chaim_Weizmann.
A query in very true sense. Let me first tell you that it is not possible to answer that you are interested in. I suggest that you go to a BIOINFORMATICS database called KEGG where you will see several pathways when you will put up this question in the subject window. There you will get the different enzymes involved. Now next try to get some information about these enzymes (specially about their regulatory aspects). This will answer your question as precisely as you require it.
Dear Zhongping shi, is it possible to share the isolation process of Clostridium acetobutylicum from mixed culture (like anaerobic sludge).
- Badges
- Science topic
- Similar topics
- Chemistry
- Biochemistry