How to develop Normative Value? What are the process/steps/methodology ?

Hello RG friends, I am planning to develop new normative Data (Value) for one of gait parameters but i dont get such idea about the process/steps/methodology to develop that. So kindly help me for...

09 October 2018 5,411 3 View

How to measure Knee Loading during standing and walking..?

Plantar pressure gives a overall weight load during standing and walking but if we want to measure only knee loading than how can we measure it ? Because in many conditions and activities knee...

05 June 2018 1,234 0 View

Is anybody using Zebris FDM 1.12 force platform for measure plantar pressure distribution and COP.....?

I am planning to do one research on plantar pressure distribution among elderly population. But i don't get any evidence regarding validity and reliability of Zebris FDM 1.12 force platform tools....

02 March 2018 3,688 0 View

Does Sample Size really affect on result of research..?

Sample size is very subjective in research. Many of think in a different way for the same research project. Even in articles, very few author clearly mention about the sample size calculation and...

01 February 2018 519 10 View

Is anybody have a Health Related Quality of life, Satisfaction with life Questionnaire....?

Hello all I required two Questionnaire that is Health Related Quality of life, Satisfaction with life Questionnaire with its calculation. If anyone have than plz send me on my Email ID:...

11 December 2017 1,698 0 View

How can we believe on someone's research....?

Number of research paper published every day among worldwide. Even for the same research many articles was published with different conclusion. But my question is how can we believe on someone's...

06 July 2017 8,904 0 View

How to correct lumbar flat-back?

Lumber flat back is major problem with lower back pain those who are prolong sitting position work and because of that they have a lower back pain. So is there any treatment to treat that or even...

02 March 2017 3,194 3 View

Are there any methods or criteria for calculating the Sample Size Calculation for Research?

Now -a-days most of the researcher added that they did the Sample Size Calculation but they didn't show which method they are doing. For the same study, sample size calculation is different from...

11 December 2015 7,390 8 View

What is the reason to avoid the early weight bearing in patients with fracture of neck of femur....?

11 December 2015 9,165 0 View

Whether squatting is indicated in knee osteoarthritis individuals?

I am dealing with participants with OA knee in attempt to control their symptoms and to improve their knee function. Any suggestion or articles are welcome.

08 September 2015 2,774 1 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View