Is there any protocol for mitochondria isolation from human or mouse sperm?

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How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

Gromacs first step of minimization ?

I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...

07 August 2024 2,598 1 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Normality assumption for linear regression is The assumption of normality is whether for residual errors or predictor variavble?

When we conduct linear regression, there are several assumptions. The assumption of normality is whether the residual errors are normally distributed, not whether a predictor is normal?

31 July 2024 6,164 3 View

In SEM, if a latent variable consists of three observed variables, and each observed variable has four items, what should I do?

Suppose I am studying the construct of "Academic Motivation" (latent variable) in students, which is measured through three observed variables: "Intrinsic Motivation," "Extrinsic Motivation," and...

31 July 2024 3,913 6 View

Data Gathering for Analyzing the Relationship Between Student Bullying Experiences and Other Variables?

I want to analyze the relationship between a student's experience of bullying and other variables using regression, the Process macro, or SEM. 1. Should I recruit both students who have...

30 July 2024 620 1 View

Can I Use D-PBS for Preparing a 10% Sucrose Solution for Lentivirus Concentration?

Hello, I am a master's student currently conducting lentivirus transduction experiments in Jurkat cells. The virus is not effectively entering the Jurkat cells, so I am planning to use an...

21 July 2024 4,676 4 View

What should be consider when do CVD?

I'm trying to create a new substance. Are there any important considerations when fabricating with CVD? For example, lattice constant If you have any such considerations, please let me know.

15 July 2024 9,359 0 View

Please inform me about the International Conference on Plant Biology?

Please inform me about the International Conference on Plant Molecular Biology, or Plant Biology, scheduled for December 2024, January/February, or March 2025.

14 July 2024 3,120 7 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?

02 August 2024 9,518 2 View

How Can I Apply Hashin Damage Properties to Solid Elements in Abaqus Without Using VUMAT?

Sure, here is the translation: I have a 3D orthogonal woven composite structure where warp, weft, and binder yarns are oriented in three directions. After modeling these yarns and the matrix in...

28 July 2024 8,169 0 View

Will the catalytic effect of retained austenite happens in steels including RA?

The catalytic effect of retained austenite refers to the phenomenon wherein quenching steels with a small amount of retained austenite, when subjected to tempering at a specific temperature...

27 July 2024 8,508 0 View

Reason for discontinuities in my Band structure?

Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...

25 July 2024 2,920 11 View

Best method for extracting RNA from morulae?

Best method and number of embryos for extracting RNA from pig morulae?

21 July 2024 4,398 1 View

How can I calculate spin texture using Quantum Espresso for non-colinear case ?

I want to calculate the spin texture for the electronic band structure obtained from SOC calculation. Is there any way for calculation of spin texture using quantum espresso??

19 July 2024 8,571 1 View

The reason for breeding Loxp mice with FLPe mice?

I am planning to use CD4 Cre mice crossed with Loxp mice. However, the laboratory from which I am trying to get the loxp sperm says they have "the product of the EMMA ko crossed with the FLPe to...