Seung Hyun Park

4 Questions 6 Answers 0 Followers

Questions related from Seung Hyun Park

The addition of ATP is required for Co-IP or pull-down assay?

Hello. I'm working on check protein-protein interaction. My hypothesis is protein A (kinase) phosphorylates protein B. So I tried lot of Co-IP bur failed. So I planned to pull-down assay using...

07 May 2022 3,631 2 View

I want measure intracellular pH change in sperm with BCECF-AM. It requires pH standard?

This is my first time to try measure intracellular pH but it is confusing about protocol. I have BCECF/AM (216254, CAS 117464-70-7, Sigma) and i understood protocol briefly as below. 1. Load...

05 June 2020 6,460 6 View

Is there any protocol for mitochondria isolation from human or mouse sperm?

I'm looking for protocols about mitochondria isolation from sperm. However, many protocols are applicable to tissues or cells. Spermatozoa have a different cellular structure compared to other...

02 April 2020 5,155 4 View

How proteasome inhibitor (MG132, MG115) works?

I'm now studying proteasome and proteasome inhibitors such as MG132 and MG115. I studied MG132 is a specific inhibitor of 26S proteasome but MG115 can inhibit both of 26S proteasome and 20S...

03 March 2020 9,486 2 View