i need to recover or extract DNA from agarose gel is there any protocol for doing so ?
Borders are markers of nations. Do cartographical decisions involve emotions especially partitioning a unified territorial region ?
29 April 2024 3,715 5 View
I am trying to finfd electric, magnetic field and surface current but i am getting this error. not able to find result.
19 January 2024 1,467 2 View
I am trying to design meta-material absorber using CST. I am getting meshing error "Could not find a mesh node while processing lossy metal sheets. Click here to show the positions. Please...
04 September 2022 235 0 View
Hello all, Can anyone help me with how one calculates the p-band center and what is the significance of it. For photocatalysis applications it is observed that p-band theory is helpful in...
10 April 2022 3,251 1 View
Hi everyone, I am working on CD thermal melts for a protein high in alpha-helical content. At high temperatures, I see the loss of one alpha-helical dip at 208 nm (image attached). I would like...
15 March 2022 9,849 3 View
Hello! I am titrating proteins into polystyrene nanoparticles, using ITC. Initially I get good heats evolved which gradually decrease upon nanoparticle saturation. However, just before saturation...
04 October 2021 10,015 3 View
In general discourse, waste to energy as a means of waste management scores poorly in policy discourse in governments. But, the real cost of waste is actually way more than the cost incurred in...
11 July 2021 7,109 9 View
I tried transforming my Ligated gene into the competent cells but nothing grew at all. So when we finally had some colonies, we did plasmid miniprep to increase the concentration. We need more...
19 April 2021 8,053 4 View
Recently we have installed Gamma spectrometry set up at our institution. It has NaI flat type detector of size 3"x3". To analyze soil, sand, rock, and other environmental samples we require...
29 March 2021 971 1 View
Hello All, I am currently modeling a solenoid on Matlab. I have evaluated the inductance of the solenoid based on its geometrical configuration using the formula, L = mu0*n^2*V where, mu0 =...
16 February 2021 5,793 5 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
or can you recommend articles showing the differential effect of LPS in a time dependant manner?
28 July 2024 2,547 1 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...
17 July 2024 6,213 4 View
I start with IDT ordered 124 bp ssDNA that is PCR amplified, then I use a QIAgen agarose gel extraction to get my exact product, and I'm trying to purify ~95bp RNA by denaturing PAGE to prevent...
16 July 2024 4,604 0 View
So - my lab did a bunch of RNA isolations from tissue culture cells, for purposes of RNA-seq, and submitted them to a core facility. We got back a notification that the RINs were too low, and we...
14 July 2024 641 1 View