Is there any problem detected with the new drug 'Darunavir' a HIV-1 protease inhibitor?

More Anjaly Thambi's questions See All

Calculating fold increase?

Dear All, We work on a project with aims looking at the changes brought about by ketogenic diet( a special form of diet found to be effective in treating drug-resistant epilepsies). We have...

25 December 2018 9,551 3 View

Can we apply t-test directly to Ct values obtained from real-time PCR?

Our study comprises of PCR analysis of DNA isolated from fecal samples at two time points (at the baseline and after intervention). We have carried out PCR and have obtained the Ct values....

25 November 2018 1,583 7 View

Can anyone please get me the reaction of trans-1-bromo-4-tert-butylcyclohexane with iodine?

Nucleophilic Substitution reaction

12 November 2012 9,788 5 View

Acidity and pKa

We can measure the acidity of compounds by its Ka value. Then, what is the necessity of finding pKa value? Why there is a term called pKa?

15 October 2012 7,521 3 View

Why do only certain nuclei show magnetic resonance?

15 September 2012 8,995 5 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Hi everyone, I want to lower the pH of microbiological broth, what are the best options?

I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.

03 February 2021 6,959 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

How do you quantify bacterial qPCR data?

Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...

01 February 2021 7,153 4 View

How can I know the stationary phase of growth of several bacteria?

How can I know the stationary phase of growth of several bacteria? We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the...

31 January 2021 4,528 10 View

Helmi Sulaiman

U r welcome.. Darunavir was introduced in my centre few yrs ago as a study drug and the study has ended.. I don't think it has any generic form leading to a very expensive cost for the patient to be on the patented drug i.e. prezista. But I do have a feeling that this PI like other PI (exc atazanavir) has metabolic complications on lipid profile and PI is well known for drug drug interaction