I have prepared Gelatin-chitosan-Bioglass composite 3D scaffold for orthopedic application. I need to show the internal interconnected 3D porous structure of this scaffold. Is there any method other than Micro CT scan??
For a 3D view of your samples surface you could use scanning electron microscopy - SEM, otherwise for a whole view, in my opinion, micro-CT scan still remains the best option
micro-CT is a good way to get the whole picture of scaffold topology, but the resolution may not be enough to reveal details on the scaffold inter-surface
beside that, you can try:
1, SEM of cross-sector of the scaffold
2, stain your scaffold with FITC or PI, and using confocal or 2-photo microscope to reveal its topology in WET status (which could be very different to topology in DRY status), confocal can penetrate insider solid ~ 200 micrometer.
3, mercury intrusion to reveal the porosity and pore distribution range
Hope it can help
i had used FITC and PI staining for obtaining the 3D view of the Cell's on the scaffold but will it also work for staining the scaffold material?
It also work, using right linker to attached dye on your material surface, and wash the scaffold carefully to remove un-attached dye
micro CT may be the only method to actually scan the entire scaffold in 3D - without destroying it. You should first have a good idea how small /large features you need to analyze. There area scenners that can reach micron resolution or image even nanoscale features. However, then your field of view is getting small and the imaged volume is not anymore necessarily representative to the entire sample.
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