Protein instability may be due to protein lysis by various factors, if we are able to measure the lysis we can understand the how and what affects its stability
I'm currently analyzing the intestine using villin CRE knockout mice. While my team specializes in liver research and we're expanding into intestinal studies, we're not very familiar with...
18 April 2024 3,776 1 View
Dear ResearchGate community, I have a statistical question which has given me a lot of headache (statistics usually do, but this is worse!). I have designed a survey in which participants read...
30 April 2023 8,369 9 View
I am modeling an underground structure with fixed support conditions around the exterior walls. I would like to apply different ground acceleration time history data into specific nodes (joints)...
05 November 2022 1,304 2 View
Dear all, I'm on the research, based on stochastic frontier approach (SFA), Battese and Coelli time-varying model (1995). The study has the purpose of estimating technical efficiency and...
19 December 2021 399 0 View
We are planning an evaluation of an intervention that is designed to increase kindergarten staff's ability to identify and manage concerns of child maltreatment (i.e., maltreatment of kids...
19 October 2021 3,680 3 View
Hello, I am a beginner in ns3. I would like to add an energy module to the UE node to check the battery changes of the UEs. By the way, it seems that ns3 only supports wifi energy module. Does...
06 March 2021 6,261 3 View
Any trick or tip to make ECC algorithm white box secure.
08 December 2020 714 1 View
Hi folks, I wonder if anyone know how to calculate the fractional vegetation cover (FVC) from Landsat imagery? As far as I have read about that NDVI, NDVIs, and NDVIv are three required input...
07 December 2020 4,394 2 View
I wonder if anyone know how to calculate the Potential solar Radiation (PSR) & Topographic Wetness Index (TWI). in R? I had DEM imported in R already also with slope, aspect and Topographic...
18 November 2019 7,683 3 View
Please suggest some Open source Testbeds
12 March 2019 4,255 4 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View