Is there any manual protocol (without kits) to proceed ATP assay for cell survival?

More Liebig Debnath's questions See All

Does continues partial charging/discharging do any harm to the BESS performance and degradation?

For a grid connected BESS (lets say 100 MW/200 MWh system, LFP battery), if we charge the BESS in such a way that whenever we can access power from the solar that will charge the BESS. Hence it...

01 August 2024 9,903 7 View

I am interested to learn Transfer matrix method for perovskite solar cell. Any suggestions how to get a knowledge of this?

Theoretical method for perovskite solar cell

12 March 2024 9,329 0 View

Shear force application ?

How to apply shear force of a 3D block .can anyone provide Lammps input script on the application of shear force between two different fixed layer of a block?

31 January 2024 9,025 1 View

Good Leader Member Exchange is the solution to all kind of organizational cultural requirement. Comment on its involvement in Agile environment !?

Hint: Explore how good Leader-Member Exchange (LMX) can positively impact organizational culture by fostering strong leader-subordinate relationships. Discuss its relevance in the context of Agile...

09 January 2024 8,454 2 View

Why BET surface area increases after adsorption on wood straw biochar?

I have implemented an adsorption test of heavy metal solution using wood straw biochar(without any Acid/Alkali activation) as an adsorbent. The degassing temp was 200C for 6h. After adsorption,...

13 December 2023 5,168 1 View

Are there reliable platforms to publish mini reviews in biology?

I am looking for a platform to publish a mini-review, suggestions, please.

22 September 2023 4,392 0 View

Why am I getting forward conversion rate coefficients to be Zero (0) in a MESMER simulation?

I am getting a rate coefficient (Barts-Widom Phenomenological) to be zero for a unimolecular decomposition reaction. Similar reaction with another conformer of the same molecule gives some rate...

04 July 2023 2,963 2 View

How to get the reaction path from the Polyrate output (.fu27) file using XMOL package?

I am trying to visualize the change in the molecular geometry along the reaction path using the output from the Polyrate17-C package for a gas phase addition reaction. As mentioned in the Polyrate...

19 June 2023 5,168 0 View

Can I continue cell culture with e14t2ga embryonic stem cell lines without the feeder layer, specially in 0.1 to 1% gelatin ?

Feel free to help me figuring out.

28 May 2023 4,658 0 View

What is more important while computing descriptive statistics- mean or replicates?

While computing descriptive statistics should I use means or replications.

16 February 2023 1,165 6 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View

How to assess the viability and manage debris in fish primary cell cultures?

Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...

10 July 2024 4,918 2 View

What factors could be contributing on low tumor take in female Nude mice?

Hello everyone, I'm working with a PA-1 ovarian cancer cell line xenograft model in female nude mice. My cells are at 372 passages, the cell repository bank themselves gave at 352th passage....

05 July 2024 5,860 1 View

Subin Kim

Look up the kit that you have

Liebig Debnath

@subin Kim I don't have any kit. I want to do it manually without kits. That's why I asked for manual protocol for ATP assay.

I see, in that case I am unsure. You will likely need luciferase though if you are making your own solutions/buffers.

Ok sir. But please kindly give some response if you will find any information or references about this. Thank you for your response sir.