What colour is a resorufin solution at pH < 6.1?

The redox dye resazurin (dark blue) can be reduced in aqueous solution at pH > 6.1 via resorufin (pink) to dihydroresorufin (colourless). What colour is the resorufin solution at pH < 6.1?

21 January 2021 8,442 2 View

We use Thermo Scientific Superfrost plus gold coated slides. Why our cryo sections of dry fruits do not stick to the slides?

We use Thermo Scientific Superfrost plus gold coated slides. Why our cryo sections of dry fruits do not stick to the slides?

13 January 2020 1,215 1 View

My mysterious red dye may not be neutralized before HPLC/MS as it is only stable between pH 12.5-12.9. Who could help with equipment tolerating pH13?

More information in https://www.chemistryviews.org/details/education/10821368/150_Years_Alfred_Wohlk.html

04 July 2019 1,409 1 View

Are smaller insect species more sensitive to residual insectides?

Alarming declines of insects elicited a vivid public debate, particularly in Germany (#Insektensterben), but also in many other countries (see...

06 September 2017 7,975 4 View

Someone knows a company that offers gene synthesis in expression plasmids for use in plants?

16 May 2016 8,212 3 View

Which flower signals are honest?

There are some reports about honest floral signals, e.g. flower colour change in many flowering plants correleted to nectar production, size of Dalechampia bracts indicating the size of a resin...

25 April 2016 1,418 5 View

Which pan trap colors are attractive for bees?

Dear All, I want to learn about pan trapping of bees and the most attractive colours. Does anybody know published spectral reflectance curves of pan trap colors? Can anybody explain the term...

08 March 2016 7,710 44 View

How does flower suntracking correspond to bee foraging behaviour?

Many flowers show heliotropism and the flowers are sun tracking. The discussed benefits of sun tracking flowers are heating up the flowers for better pollen germination and seed production, and...

26 January 2016 9,316 5 View

Does it make sense to compare linear regression slopes in the Murmansk region (Russia) using air temperatures from gridded E-OBS data?

12 June 2015 8,483 14 View

Why do some researchers neglect multiplicity in the calculation of statistical significance?

14 February 2014 786 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

Any stable dye to color acrylamide hydrogel beads?

Hi! We are generating hydrogel beads(50-60um) using microfluidics as single-cell beads. To simply all the experiments (especially the centrifuge), I am looking for a dye. The dye should be stable...

14 January 2021 4,383 8 View

Compare to prevalence before and after covid in population studies?

Dear researchers, I have a prevalence of some diet habit in relatively large narrow population of children 13 years old in 2019 and 2020. Logically they are different population. However, since...

10 January 2021 3,170 9 View