How to identify and retrieve many (+30) RNASeq datasets (Fasta) under a specific criteria in a practical way?

22 March 2020 3,649 3 View

What have been the contributions of cultural studies to the teaching of English as a second or foreign language?

the idea that intercultural competence develops communicative competences. Worldwide, this relation intercultural-communicative competence has been studied by teachers and researchers (Agudelo,...

01 February 2020 9,056 3 View

How to conduct a Chi-square analysis dealing with two independent groups?

Hello fellow scholars, I would like assistance with a Chi-square analysis dealing with two independent groups. In one research article I was reading, the researcher was studying US attitudes...

25 January 2020 7,039 6 View

How is the Linguistic Colonialism evidenced in the English Textbooks of Multinational Publishing houses in teaching of English as a second language?

The purpose of this question is to examine and compare the concepts of linguistic colonialism and cultural alienation in University textbooks for teaching English as opposed to the theories about...

01 January 2020 1,603 3 View

How to connect beam188 and link180 elements in ANSYS APDL?

I am currently working on the design of a deployable truss structure and I would like to know what is the proper way to connect beam188 and link180 elements in APDL? The diagonals (purple lines)...

07 April 2019 3,152 4 View

What's the amplified gene in the DNA ladder assay for apoptosis detection?

Greettings, I need some guidance. Regarding to the DNA ladder assay for apoptosis detection which is based on electrophoresis analysis, I would like to know what is the gene to be amplified in...

03 April 2019 9,559 4 View

Protein misfolding behaviour?

Hi all, I am working on a tricky protein. I surpassed some initial aggregation behaviour by adding 0.3% CHAPS and 1mM TCEP, buffer in which is happy until the removal of the SUMO tag, then it...

09 December 2018 9,460 3 View

In a Real Time PCR, is a CT of 4.6 (average) enough to determine presence of babesia/anaplasma in a sample?

We are currently trying to identify presence of babesia bovis, babesia bigemina, and anaplasma marginale in DNA isolated from white blood cells. However, the amplification plot values start...

12 November 2018 4,051 3 View

Hi everyone, someone have made real time PCR in tissue fixed ?

for us is very importan the purifiaction and extration of the mRNA in tissue previusly fixed with paraformaldheide, thats possible? or may be we can used other solutions for preserve the sample,...

04 May 2018 8,912 0 View

Does anyone know a clustering algorithm/software that transforms a group of images into an undirected weighted graph?

Hi everyone. I'm currently trying to assess the ability of human volunteers to cluster a set of images into a fixed number of clusters according to perceived visual similarity (the images are...

14 January 2018 8,222 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Are there SDMs that can take in percentage data?

Hi everyone, I am wondering if anyone knows of a species distribution model that can take in percentage data (i.e. presence data with prevalence values attached to them)? The dataset I have...

18 February 2021 7,312 3 View

What is the correct common name for Hydrochoerus hydrochaeris?

There are two extant species of Hydrochoerus: Hydrochoerus isthmius, the lesser or Panamanian capybara, and the genotype species H. hydrochaeris. The latter is the more common species of capybara,...

11 February 2021 3,494 14 View

Loss of biodiversity its impact on terrestrial ecosystem?

I need research papers with problem statement as well as research questions in respect of the above mentioned topics

04 February 2021 2,240 15 View

Is it possible estimating environmental conditions with a Bayesian approach?

In ecology specific species can be used to get an indication/estimation of the environmental conditions. A simplistic example, lets assume I find a pike (Esox lucius). We know this species does...

01 February 2021 10,034 4 View

How the microplastics levels in freshwater and marine water ecosystem be controlled?

Microplastics have been found in land, air, freshwater, effluent from a wastewater treatment plant and even in tap water and bottled water. The small plastic particles (less than 5 mm) pose a...

19 January 2021 8,637 3 View

Why are my Maxent SDM projections coming up almost entirely blue?

Hello Maxent Community, I have been generating SDM models and projections in Maxent. I have had some success with one set of variables (some Hydroshed variables mixed with World CLIM variables);...

19 January 2021 3,994 7 View