How to detect spurious correlation in a statistical dataset with binary variables?

17 January 2021 7,892 25 View

Can somebody explain to me why RNA looks like this on RNA electrophoresis picture down below?

I extract the RNA from muscle tissue using Qiagen RNeasy Micro kit (50) (kat.74004). Everything fine with RNA concentration - about 300-500 ng/microliter. Before putting it into wells I mix my RNA...

19 November 2020 2,449 5 View

Can you help me figure out how many EDTA I need to add in the solution?

Composition 2X Loading dye for RNA electrophoresis 95% formamide 0.025% SDS 0.025% bromophenol blue 0.025% xylene cyanol FF 0.025% ethidium bromide 0.5 mM EDTA. I wanna to prepare 10 ml of the...

10 November 2020 4,759 3 View

RNN/LSTM Networks : How to take into account different values of times on different time-series?

LSTM tools of the Matlab allow us to perform classification of the time-series. It is assumed, that different time-series can have different length, but they should have same interval of...

13 October 2020 3,484 3 View

Deep Learning : How on one model to take into account both dynamic (time-series) and static features ?

Matlab tools for Deeep Learning allow us to solve classification tasks with static features (e.g., pixels values of images) and tasks with dynamic features (time-series). For time-series it is...

06 September 2020 9,942 6 View

How to apply Operational and Dynamic Routines to Organization Design?

Dear colleagues, I'm working on methodology of transforming existing organization for manufacturing companies. Could you please guide me on how to approach the following questions: 1. How to...

08 July 2020 1,754 7 View

How to perform the ordinary one-way ANOVA analysis to compare the pharmacological effects for different drug-like substances?

The one-way ANOVA analysis is important to find out whether measured pharmacological effects (the means and medians) observed for three or more drugs are distinct or they are due to a chance....

11 May 2020 4,605 6 View

How to evaluate two-dimensional probability based on two one-dimensional (marginal)?

23 April 2020 6,418 9 View

Does anybody have experimental data on ferroresonance phenomena?

Does anybody have a reliable experimental data on ferroresonance phenomena in a transformer (single- or three-phase)? Thanking you in anticipation, Sergey Zirka

17 April 2020 8,656 4 View

Are there any changes in the tensional behavior of single-wall carbon nanotubes (CNT) with different chiral indexes?

We simulated (LAMMPS program) the tensional behavior of CNTs with different chiral indexes (10, 10; 0, 10, and 10,0) using the original CH.airebo potentials (Stuart et al., 2000). The plot...

16 January 2020 8,147 3 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

Is that possible to do an impedance measurement without FRA module?

One of my colleagues said, "the impedance you can measure with the three electrodes and with the specific sequence varying the frequency" Is it possible to configure the potenciostat without the...

21 February 2021 418 2 View

Choice of Ab for Co-IP: Does an anti-(tag) antibody (such as anti-flag/ anti-myc) a must if I am using co-transfected cells for Co-IP?

Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...

16 February 2021 9,050 5 View

How to show in a scientific paper format the post-hoc test of 4 variables involved, when 3 by 3 variables are significant?

In a study involving 4 variables (species(2x), temperature(2x), nutrients(2x) and light(3x)), 3 by 3 variables, for example, "species", "temperature" and "nutrients" are significant. I end up with...

15 February 2021 8,018 2 View

How to mesh complex geometry?

I have been trying to mesh some complex geometry and have been having issues. I have attached images of the geometry in question. This structure is very large in size but has some comparatively...

05 February 2021 6,379 5 View

STS Score - Excel sheet calculator?

I'm looking for an excel sheet to calculate the STS Score. From the website http://riskcalc.sts.org/stswebriskcalc/calculate. Does anyone have a worksheet to calculate the STS Score?

25 January 2021 958 4 View

How can I do antigen retrieval for proliferative markers (MCM2, Ki67 etc) While preserving a sensitive endogenous reporter?

I am trying to do antigen retrieval on mouse brain sections to stain for proliferative markers like MCM2, Ki67, PCNA etc on tissue that has mCherry conjugated to a transcription factor of interest...

18 January 2021 1,023 3 View

Does anybody have experience in expressing and incorporating two proteins (to express as a protein complex) in one cloning site?

I want to clone have two proteins cloned into pPROexHtb+ (which has one cloning site). I want to express them as a protein complex. My question is would you generally put a stop codon after first...

09 January 2021 8,811 2 View

What to do with this Gaussian Convergence error?

Hi! I'm kind of a beginner with computational chemistry and I\m trying to study the interaction between copper ion and a graphitic carbon nitride quantum dot. the calculation converges with HF but...

02 January 2021 8,684 5 View

How to find the squeal frequencies in the Abaqus?

I am analyzing the squeal of simple brake using Abaqus and I tried to find the unstable frequencies using complex frequency which caused the squealing noise; however, all of the modes have nearly...

01 January 2021 7,035 4 View