How can I prevent gene expression before collecting microorganisms by centrifugation?

Hy everybody, I am looking for a way to stop gene expression during harvesting of microalgae to ensure reliable RNAseq in response to temperature. I am affraid working on ice and performing...

25 November 2020 7,282 3 View

How to increase the efficiency of overexpression using lentiviral TET-ON system in primary kidney cancer cells?

I am using a lentiviral TET-ON system for conditional overexpression of my gene of interest in primary kidney cancer cells. I use viral load from transfection of HEK293T cells after 48 and 72...

16 November 2020 2,496 1 View

Has any endophytic microbe been isolated from the bark of coffee plant?

Any bacteria or Fungi from the barks of coffee

10 September 2018 2,993 4 View

Very minimal researches published on online businesses even though online businesses is gaining moment world wide due to ease access of the internet?

Very minimal researches published on online businesses even though online businesses are gaining momentum world wide due to ease access of the internet?

22 May 2018 4,022 3 View

What is the maximum solubility of RNA ?

I am looking for publications (or patents) stating the solubility of RNA (either total RNA, tRNA, whatever the species) in water or in saline solutions. Thanks for helping me !

19 September 2017 9,880 5 View

Can someone provide a protocol for building yeast strains using Tet-Off expression system?

I am trying to express my gene of interest using a Tetracycline inducible vector. Although I need my gene of interest of be off and only be turned on for a short period of time when needed, I only...

07 July 2017 3,994 19 View

How to troubleshoot qPCR primer optimizations?

I am having some issues with primer optimization for qPCR. Typically, I first test various concentrations of primers to figure out which concentrations gives the best melting curves and the lowest...

17 January 2017 9,923 2 View

Xylan from beechwood discontinued by Sigma-Aldrich

28 August 2016 3,993 7 View

Hi! can anyone help me identify this plant from a lake in the Philippines which is constantly being affected by fish kill?

07 January 2016 2,793 3 View

Do plasmids need to be CIP treated for plasmid religation assay?

I am trying an experiment in which I measure the NHEJ efficiency of a mutant yeast strain and compare to wild-type to analyze whether the mutation affects the NHEJ process. I take a low copy...

16 December 2015 2,586 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Image Processing and classification using keras/Tensorflow?

Do you have some recommendations approaches on Image processing and classification of plant diseases using TensorFlow/Keras?

02 March 2021 1,493 4 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

Can anyone please help me in processing SAR SLC data to generate interferogram using SNAP?

I have two scenes of the study area, one top and the other bottom. As per the tutorials and papers published, the step include coregistering the master sand slave images. However, when i try to...

24 February 2021 6,372 1 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View