Is there an endonuclease that has decreased sensitivity for tRNAs?

16 October 2023 1 8K Report

Hi all,

I am trying to do ribosome footprints with an RNAse that has decreased or no sensitivity to tRNAs. Does anyone have a suggestion? I would be grateful for someone to share their expertise.

Many thanks in advance!

Dhrubo Sikdar

maybe a mutation in the tRNA sequence would cause a endonulease to not be effective enough? see if this article helps Article How mutation in tRNA distant from the anticodon affect the f...

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