Dear Fowad. As student I assisted a research where we analysed VFA from silage extracts and from rumen simulation systems using HPLC. Also, is it possible to use some titrations. Please see the following links

https://www.researchgate.net/post/Best_method_to_find_Volatile_Fatty_acids_VFA_without_GC_and_HPLC

Article An HPLC method for estimation of volatile fatty acids in ruminal fluid

Thank you Mr. Luis. Yes I read that HPLC can be used to estimate VFA, But also we have problem with the apparatus and the spare parts, I search for a possible non-chromatographical method.

Then, see tritation methods on the first link

Dear Fowad, we use GC for VFA analysis. You can read a comparative study of different technical procedures for VFA analysis in the following web address:

http://www.wseas.us/e-library/conferences/2009/lalaguna/EPREWA/EPREWA77.pdf

as a total VFA you can use steam distillation 

You could use GC for VFA analysis or steam distillation for determination total VFA

Thank you dear Jalal

Dear

Total vfa can be easily calculated by distillation method and cooling the distilate on ice pack. But if you want to calculare the proportion of individual long chain short chain and CLA the high precision instruments are required. While using these instruments yoi have to extract the vfa from rumen liquor and you have to run in GC or HPLC. Depending on their molecular wt and chain length they will have peaks at different retention time. Bu seeing the area of peak in GC we can calculate its concentration. It is a routine analysis now a days. Protocol is easily available. If you need i wl give you.

Regards

Partha

agree with Partha

Dear Fowad

Distillation method is widely used to determine TVFAs in rumen liquor. The suggestions given Dr. Partha. you easily follow.   

thank you Dr Taha and Dr Nathu Ram

Dear Dr.  Partha Sarathi

I was a student of HAU, Hisar, under the Deptt. of  Animal Nutrition, Dr. K.K. Sinhgal was my Thesis Examiner, Dept. of Dairy Cattle Nutrition and I many of your Teachers working in NDRI, including your Present Director. So, I feel proud to learn a lot from your country. Possibly, You are also a nutritionist, your suggestions are absolutely correct.   

Dears: Partha, Taha, Nathu

Your contribution is highly appreciated. 

Dear dr nathu. 

Thank you for your words. I belong to dairy cattle nutrition division of national dairy research institute. A phd scholar in animal nutrition.

Dear Dr. Fowad and Partha

Among we all belongs to scientific community, so, we  will definitely share our knowledge and experiences to the peoples working in the globe for the betterment of the farmers who are depends on livestock for their livelihood. 

Dr.  Partha at present two of my junior colleagues have joined in an international Training Program related to use biotechnological in  FnAGR conservation. 

Thank you