Is there a website that allow searching for homology of a short sequence (such as BLAST) against expression plasmid only?

More François-Xavier Campbell-Valois's questions See All

Anyway to compare data with two different sample sizes?

Hi all, I'm looking at accelerometer data. I used two accelerometers at once for a backup, but now I need to correlate the two datasets provided. I'm only looking at one axis on each, but one set...

14 January 2020 4,525 3 View

S there a web based application to generate prokaryote chromosome map from a Genbank entry?

I am simply looking for a website that will allow generating a graphical map of a prokaryote chromosome (>4Mb) or large plasmid from a Genbank reference number. Any suggestions very much...

10 November 2017 7,427 2 View

What is the shortest antibiotic resistance gene that you know of ?

We would like to work with very short antibiotic resistance gene that are useable as selection marker in the bacteria Escherichia coli and potentially other Gram-negative strains. The shortest...

05 June 2017 527 6 View

What is the best recent textbook about mass spectrometry (with focus on biological samples analysis) and/or proteomics that you would recommend?

I am looking for a recent and excellent textbook on these topics.

05 June 2017 1,374 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any suggestions for a website that allows you to create questionnaires and gather unlimited data (Totally free)?

I have found only four of them; www.surveyplanet.com www.online-anket.gen.tr www.surveey.com and of course google forms. Expecting to see your suggestions if there is any. Thank you

28 February 2021 3,237 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Hugo Germain

NCBI has a tool designed specifically for your purpose. It searches your sequence for any vector sequences. It is used to clean up raw sequence directly out of the sequencer, so it performs very well on short sequences. Here is the link:

https://www.ncbi.nlm.nih.gov/tools/vecscreen/

François-Xavier Campbell-Valois

Excellent ça marche bien! Merci beaucoup.

Translation for visitors: tested and worked well! Thanks