I am western blotting and want to use and old aliquot of an antibody we no longer have. The milk had NaN3 in it so it's not sour.
If your antibody is in milk and it was frozen, possibly it is still active does not matter if there were added NaN3. You can use directly the milk as media for incubation if it contains the first antibody you need for western blot, just dilute x500 or x1000 before incubation. After washing, no any NaN3 added will be there and I suppose you have a second antibody bound to peroxidase or phosphatase. If the question is that you want to isolate the antibodies in milk, just use a protein A or protein G chromatographic column.
I doubt that a purification attempt with Protein A/G will work well, as milk contains bovine IgG, thus competing with your AB of interest for the resin. At least, you have to take this into account and depending on the capacity of your Protein A/G column do repeated extractions until all IgG has been recovered.
Endogenous milk IgG level is not high and I suppose the antibody concentration is higher if it was previously prepared for incubation as it appears from NaN3 addition.
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