Hello everyone,
I am hoping to observe adhesion microscopically using staining. I have been doing adhesion experiments on 96-well polystyrene plates but I want to be able to visually observe the attachment.
Has anybody successfully done this? What type of microscopic slide should I be looking for?
Thank you for your help.
Hi Valerie Diane Valeriano,
Were you able to identify a suitable method to assess the adhesion of commercial mucin to microscope slides/96-well immunoplates?
If you can shed any light on this, I would really appreciate it as I am having some trouble with the binding of mucin to my plates.
Thanks,
Ashwin
Hi Ashwin Ramesh ,
I have had communication with another researcher regarding the procedure, with which he checked with BCA.
For both of us, what worked was to bind commercial mucin to a NUNC maxisorp plate using acetate buffer (pH 5.0). A drying step was utilized after incubation with the mucin.
Take note that different approaches for mucin binding to the wells are different as the composition and concentration of mucin complexes used are different. But maybe the above steps may also be helpful for you, especially with the drying step.
Here is the link to our paper that details the approach we have used: Article Carbohydrate-binding specificities of potential probiotic La...
Best,
Valerie
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