I will be analyzing digital images through RGB measurements. For this, I will use Image J. I am not an expert with it so what I do is select an area (using the round selector) then measure the RGB.
Since I will be selecting several areas around the image, it will be very time-saving if I can select multiple areas and measure the RGB of each selection.
Can anyone help me with this? A special plugin or a different freeware? I appreciate all your comments and suggestions!.Thanks.
I am not sure what exactly you mean by "measure the RGB" (I haven't used ImageJ much). I am assuming that you mean statistics like mean/min/max intensities, for all the 3 channels.
Anyway, the fundamental requirement seems to be that you want to select multiple areas and measure statistics of each region separately. Try the following -
1) Open the ROI Manager, Analyze>Tools>ROI Manager. Check the "Show All" and "Labels" options in the ROI Manager window.
2) You can now use your selection tool, select a region and add it to the ROI list by clicking the "Add" button (shortcut "t") in the ROI Manager window. Repeat for the next region and so on.
3) Since you want statistics from the 3 channels separately, convert the image to a multi-channel stack by using Image>Color>Make Composite. Your image window will now contain the three channels which you can access using the bottom slider.
4) Click on the "Measure" button in the ROI Manager window. This will measure the statistics of marked regions separately and display them in the Results window. This will be for the channel which is currently displayed in the image window. Slide to the next channel, and click on the "Measure" button again and so on.
In the Results window, you might have to go to Results>Set Measurements and check the "Display Labels" option so that the results would be named appropriately (with the region name and the channel name). The labels will be blank for the measurements made before checking this option. Hence it might be a good idea to check this option after the first measurement (so that the Results window pops up), clear the results and then starting over so that you will have labels for all your measurements.
Hope this helps!
Could you specify a bit more detailed what you mean by "measure the RGB"?
Maybe you have a look at MeVisLab (http://www.mevislab.de, German website, but English language). It is a framework for image processing and well suited for repeating tasks, since it allows for creating automaticly working image processing chains. The downside is, that creating interactive (manual) processing applications is rather difficult for a novice user... However, the basic functions are available without a license, so you can check if it fits your needs.
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