28 January 2025 1 9K Report

I would like to know which proteins are expressed more during late logarithmic phase or early stationary phase of E.coli in normal LB media culture condition.

More Rahul Sharma's questions See All
Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

How does grain and grain boundary affect the ceramic when studying its dielectric properties?

I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...

07 August 2024 5,177 3 View

How prion can cause disease?

how is prion can cause disease

01 August 2024 250 1 View

Does the delta function actually make sense to use on point particles?

The delta function seems produce logical contradictions when analyzed on a fundamental level. I would be curious if anyone else agrees.

31 July 2024 10,109 3 View

What are the roles of both Monetry and Fiscal Policy coordination in optimising the Macroeconomic outcomes in the economy ?

These days policy coordination is becoming necesssary on different front. In India also it is the same case. Monetary and Fiscal policy interface is important in order to address the...

30 July 2024 2,104 6 View

IHC profiler in image j software. has anyone used it to quantify nuclear DAB positivity?

Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...

29 July 2024 2,229 0 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

Reason for discontinuities in my Band structure?

Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...

25 July 2024 2,920 11 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

How to dispose off lipids waste?

I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...

25 July 2024 637 5 View

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I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

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MAGeCK Plotting Error: Logarithmic Axis Must Have Positive Limits?

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How to reduce interstudy sample variability in RNAseq Meta-data analysis?

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RNA-Seq Data Analysis for A. thaliana: Clustering Based on Accessions?

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Exponential and Logarithm of Bounded Linear Operators Books?

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How to interpret disparities between RNAseq and q-PCR data?

Hello Researchgate community, I recently ran into a bizarre (at least I've never thought it could happen). We selected a few DEGs from the scRNA seq dataset and run q-PCR to validate the...

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What is the best method for analysing differential gene expression for only a small, targeted selection of genes (between 30-40)?

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Is it possible to analyze single nuclei RNA seq libraries with an assembled transcriptome or only with the genome?

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