Is there a solution for adhesion of P3HT:PCBM on PEDOT?

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Do you mix and add all primers together in a singel PCR reaction (4 total primers for 2 mutations) when using Agilent's multi-site mutagenesis kit?

I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...

25 July 2024 6,517 3 View

Why farmer's of India not adopting Integrated farming systems?

While IFS is beneficial

15 May 2024 4,174 4 View

What is the approach for Machine learning algorithms selection for material science?

Which Machine learning algorithms suits best in the material science for the problems that aims to determine the properties and functions of existing materials. Eg. typical problem of...

10 May 2024 1,490 3 View

How do we contruct an optimal feature set?

How do we evaluate the importance of individual features for a specific property using ML algorithms (say using GBR) and construct an optimal features set for our problem. image taken from:...

10 May 2024 2,691 4 View

What will be the effect of using the low thermal conducitvity PCMs at fixed thermal conductivity of 1 W/(m K)?

The wide application of PCMs is rather limited due to a very low conductivity (less than 1 W/(m K)). If we fix the thermal conductivity of all these PCMs at 1 W/(m K). Then what will be the effect...

07 February 2024 3,593 1 View

I have an EPDM rubber that has swelled in oil. What solvents can bring back the rubber to the original shape?

24 January 2024 7,796 1 View

Why ammonia decomposition not known on Mn based catalyst?

Why Mn catalysts are not in literature for decomposition of ammonia. There is a lot of ammonia degradation on its nitrides but not on Mn oxides. Why it is so?

20 January 2024 3,536 6 View

In the case of Metal-air battery (DFT approch), how can I find Entropy and Zero point energy?

Equation of Gibbs free energy G=∆E-ZPE-T∆S How finds the entropy and ZPE

08 January 2024 9,593 0 View

What is the impact of a Journal changing publishers for an author of a research article?

Dear all, I submitted a research manuscript to the "Journal of the Geological Society of India" on May 25, 2023. The paper underwent two rounds of "Major Revisions," which were successfully...

08 January 2024 2,822 0 View

Countess II FL Uneven Lighting. Any Fixes?

The lab that I work at uses a Countess II FL cell counter and the machine typically works fairly well. Recently we have noticed that the lighting for the brightfield is uneven and causes cells to...

28 September 2023 6,872 0 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Basis set and input instruction to calculate HOMO and LUMO using Orca for Imidazolium based organic salts?

Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...

09 August 2024 5,444 7 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Stability of the Solar System: Insights from Einstein’s Equations ?

The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...

07 August 2024 2,569 1 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View