Hello everyone,
I am going to start my master thesis on the encapsulation of insulin. In almost all protocols we found an acidic pH is necessary. Unfortunately, pH
Andrei Blasko thank you so much for your response. actually we are going to encapsulate insulin in lipidic nanoparticles. I was wondering if you could guide me about the encapsulation method in physiological pH since in our case it is impossible to go beyond 6-8 as our particles will be destroyed. is there a difference between different insulin analogs in this aspect?
I truly appreciate your time and information.
An Duong
Dear Dr. Duong,
Thank you so much for your response and your helpful guide. Actually, we are going to encapsulate insulin in lipidic nanoparticles. In our case, regarding pH, it is impossible to go below 6 or higher than 8 as the structure of our particles will be destroyed. Would you please explain if you mean simply adding a strong base to the solution when you mentioned any other pH? will the solution remain stable in another pH like it was in acidic pH?
I truly appreciate your time and information.
I am sorry that I do not actually work with insulin. I only have a little experience with lipidic nanoparticles. I am not so sure about the cases of insulin. If you prepare solid lipid nanoparticles, you may consider some references, such as Article Oral insulin delivery by means of solid lipid nanoparticles
. I think the outer phase can be buffered (pH ~6-8).In addition, I found this paper (Article Liposomes with phosphatidylethanol as a carrier for oral del...
), in which insulin was dissolved in 10 mM Tris–HCl buffer (pH 8.0).Hope that they can help you.
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