NO content analysis: Nitric oxide concentration will be determined according to the
method of Murphy and Noack (1994). Samples (5 g FW) will be incubated with 100 units
of catalase and 100 units of super oxide dismutase for 5 minutes to remove endogenous ROS before addition of 10 ml of oxyhemoglobin (5mM). After 2 minutes of incubation NO will be measured spectrophotometrically based on the conversion of oxyhemoglobin to methemoglobin by reading the absorption at 550 nm.