Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Why am I getting such large heats of dilution in my Isothermal Titration Calorimetry Experiment?

I am trying to measure binding of calcium to an expressed protein we are studying and am getting very large heats of dilution for the calcium solution in my control tests. We prepared a 1 M CaCl2...

07 February 2021 7,532 1 View

I am looking to develop a basic oral microbiome assay. Can anyone advise on culture vessel, conditions and organisms that require minimal maintenance?

I am looking to investigate oral care products with an easy-to-prepare culture of orally derrived microorganisms.

21 January 2021 3,448 1 View

Cause of blisters in ALD layer at the edge of electrode?

I've encountered a defect during processing that I can't figure out, so I'm hoping I might find some ideas here. My mask design is a microelectrode array, with 60 microelectrodes with diameter...

19 January 2021 665 1 View

Difficult to silence gene?

I have been trying to silence a gene of interest but I could not get silencing efficiencies beyond 50% My method of detection is via RT-qPCR and samples were extracted 48hrs post transfection. Are...

09 January 2021 3,404 4 View

What form of data analysis should I use for my pilot study?

I am currently conducting my final year dissertation which is looking at the efficacy of common police lineup practices. However, before I launch my study, I have put out a pilot study which is...

08 January 2021 4,376 4 View

Is it normal to use Tris buffer when testing for bacteriostatic properties of isolated compounds?

Hello, to start off I attempting to test the bacteriostatic properties of certain compounds. I bought a kit that contains some of the equipment to perform this analysis, however, in the included...

02 January 2021 2,338 2 View

Cleaning SGE Micro Channel Device?

Hello all, I have a 3-port SGE microchannel splitter in my Thermo Trace 1310 GC connected to an Isolink 2 combustion interface. My microchannel device is clogged and my flow is significantly...

29 December 2020 7,002 1 View

Weird J-V curve of solar cell?

Recently I am getting low performance (PCE and FF) and weird J-V curves on Pb-Sn-based perovskite solar cells. I have been trying to solve this for a couple of weeks now. I will like any...

19 December 2020 9,336 4 View

What is the best free corpus software for counting words?

It is a pragmatic study of tweets for some political leaders and I need to count the words are phrases used by each of them. Thanks.

15 February 2021 5,897 3 View

Difference between groups or association between variables? Can I ask both questions for a given set of variables?

I am new to statistics and trying to analyse my quantitative data. I am referring to "https://stats.idre.ucla.edu/other/mult-pkg/whatstat/" and "Laerd statistics" for the choice of statistical...

24 January 2021 4,533 4 View

Regarding to presentation of pairwise comparison of data?

22 January 2021 8,648 1 View

Eliciting target response from children, without much syntactic complexity?

We are developing a test for ad-hoc (ad-hoc) and scalar implicatures (SI) and are showing 3 images (of similar nature) to the participants: image, image with 1 item, image with 2 items. E.g....

17 January 2021 9,317 2 View

How to conduct Methodological Research?

Hi everyone, I plan to create a design for a system using previously published articles, journals, etc as a basis. I found methodological research design but I don't know how to conduct it. Like...

07 January 2021 5,824 3 View

Simultaneous equation model estimated with GMM that includes cross-section ad Fixed effect ?

Hello, I'm trying to replicate this model, but I don't understand a phrase of the authors. This is a simultaneous equation model estimated with generalized method of moments. The variables of...

21 December 2020 4,674 3 View

I have, for instance, 987 RG questions' answers. How can I go to the oldest one directly without pressing the Page-Down or using the mouse many times?

I have 751 RG questions' answers. Please help me with the followings: How can I go to the oldest one directly without pressing the Page-Down or using the mouse many times? How can I search for a...

16 December 2020 188 25 View

Is it possible to start with the phrase " Modelling of ..." for someone who is not a model developer (but is using the model made by somebody else)?

I am a beginner to DSSAT model and feeling deeply happy with DSSAT model. Most DSSAT model users begin to entitle their journal articles, book's chapter titles, proceedings etc. using the phrase...

07 September 2020 3,912 7 View

How to analyse this data on SPSS?

Hi, I am analysing the influence of cognitive and emotional response on purchase intent. I have a collection of Likert Scales (1= totally agree, 5=totally disagree) rating the phrases 'This makes...

06 September 2020 7,450 5 View

Effects of grammatical phrasing (2nd person singular vs. 1st person plural) on compliance to suggestions/prohibitions/prescriptions, etc?

Hi guys! I have a question on a framing effect-like issue. Well, everyone of us has the immediate feeling that there's a huge difference between saying - for instance - "you should respect the...

20 August 2020 7,859 6 View