Dear colleaguaes,
We need to measure the phenol compounds in pomegranate's peel. We have its dried and powdered peels. Most of the methods have used the Soxhlet but it is a time consuming method.
I am wondering if there is another method to do so!
Your responses will be appreciated in advance.
Thanks,
The total phenols were determined by Folin – Ciocalteau reagent method described by Malik and Singh, 1980 [16]. The dilute extracts of different concentrations were taken in 10ml. glass tubes and total volume made to 3ml. with distilled water these are then mixed with 0.5ml. Folin – Ciocalteau reagent (1:1 with water) and 2 ml. Na2CO3 (20 %). A blue coloured complex, molybdenum blue developed in each tube, as the phenols undergo a complex redox reaction with phosphomolibdic acid in Folin – Ciocalteau reagent in alkaline medium. The tubes containing the blue solutions were warmed for 1 min., cooled and absorbance was measured at 650 nm against the reagent blank. The standard curve was prepared using known concentrations of catechol at 650 nm. The total phenol content in the test samples was calculated from the standard curve and expressed as mg. catechol equivalent of phenol/ g. sample.
Dear Shravan M Haldhar , Amit Kumar Mukherjee
, Alaa Kareem NiamahThank you very much for your kind responses.
Best regards,
- Badges
- Science topic