Dear Andrea,
Bacto is the trademark for the tryptone distributed by the DIFCO company and should indicate a high quality standard of the product . Usually any tryptone should be ok.
Dear Andrea,
Bacto is the trademark for the tryptone distributed by the DIFCO company and should indicate a high quality standard of the product . Usually any tryptone should be ok.
Peptones
Many complex media, that is, media in which not all the specific
chemical components are known, contain peptones as the source of
nitrogen. Peptones are hydrolyzed proteins formed by enzymatic or
acidic digestion. Casein most often is used as the protein substrate for
forming peptones, but other substances, such as soybean meal, also
are commonly employed.
Below is a list of some of the peptones that are used as ingredients in
various media.
Acidase™ Peptone
A hydrochloric acid hydrolysate of casein. It has a nitrogen
content of 8% and is deficient in cystine and tryptophan.
Bacto Casitone
A pancreatic digest of casein.
Bacto Peptamin
A peptic digest of animal tissues.
Bacto Peptone
An enzymatic digest of animal tissues. It has a high concentration
of low molecular weight peptones and amino acids.
Bacto Proteose Peptone
An enzymatic digest of animal tissues. It has a high concentration
of high molecular weight peptones.
Bacto Soytone
A enzymatic hydrolysate of soybean meal.
Bacto Tryptone
A pancreatic digest of casein.
Bacto Triplets
An enzymatic hydrolysate containing numerous peptides,
including those of higher molecular weights.
Biosate™ Peptone
A hydrolysate of plant and animal proteins
Casein Hydrolysate
A hydrolysate of casein prepared with hydrochloric acid
digestion under pressure and neutralized with sodium hydroxide.
It contains total nitrogen of 7.6% and NaCl of 28.3%.
Gelatone
A pancreatic digest of gelatin.
Gelysate™ Peptone
A pancreatic digest of gelatin deficient in cystine and tryptophan
and which has a low carbohydrate content.
HiVeg Peptone
An enzymic hydrolysate of vegetable proteins that gives comparable
growth promoting properties as animal origin peptone.
HiVeg Peptone No. 1
An enzymic hydrolysate of specially selected vegetable proteins
that gives comparable growth promoting properties as
animal origin peptone No. 1.
HiVeg Peptone No. 2
An enzymic hydrolysate of vegetable proteins that gives comparable
growth promoting properties as gelatin peptone.
HiVeg Peptone No. 3
An enzymic hydrolysate of vegetable proteins that gives comparable
growth promoting properties as proteose peptone.
HiVeg Peptone No. 4
An enzymic hydrolysate of vegetable proteins that gives comparable
growth promoting properties as mycological peptone.
HiVeg Peptone No. 5
An enzymic hydrolysate of vegetable proteins that gives comparable
growth promoting properties as bio peptone.
HiVeg Peptone B
An enzymic hydrolysate of vegetable proteins that gives comparable
growth promoting properties as proteose peptone.
HiVeg Peptone C
An enzymic hydrolysate of vegetable proteins that gives comparable
growth promoting properties as animal origin peptone
A.
HiVeg Special Peptone
An enzymic hydrolysate of vegetable proteins that gives comparable
growth promoting properties as neopeptone and animal
special peptone.
Lactoalbumin Hydrolysate
A pancreatic digest of lactoalbumin, a milk whey protein. It
has high levels of amino acids. It contains total nitrogen of
11.9% and NaCl of 1.4%.
Liver Digest Neutralized
A papaic digest of liver that contains total nitrogen of 11.0%
and NaCl of 1.6%.
Mycological Peptone
A peptone that contains total nitrogen of 9.5% and NaCl of
1.1%.
Myosate™ Peptone
A pancreatic digest of heart muscle.
Neopeptone
An enzymatic digest of protein.
Peptone Bacteriological Neutralized
A mixed pancreatic and papaic digest of animal tissues. It
contains total nitrogen of 14.0% and NaCl of 1.6%.
Peptone P
A peptic digest of fresh meat that has a high sulfur content and
contains total nitrogen of 11.12% and NaCl of 9.3%.
Peptonized Milk
A pancreatic digest of high-grade skim milk powder. It has a
high carbohydrate and calcium concentration. It contains total
nitrogen of 5.3% and NaCl of 1.6%.
Phytone™ Peptone
A papaic digest of soybean meal. It has a high vitamin and a high
carbohydrate content.
Polypeptone™ Peptone
A mixture of peptones composed of equal parts of pancreatic
digest of casein and peptic digest of animal tissue.
Proteose Peptone
A specialized peptone prepared from a mixture of peptones
that contains a wide variety of high molecular weight peptides.
It contains total nitrogen of 12.7% and NaCl of 8.0%.
Proteose Peptone No. 2
An enzymatic digest of animal tissues with a high concentration
of high molecular weight peptones.
Proteose Peptone No. 3
An enzymatic digest of animal tissues. It has a high concentration
of high molecular weight peptones.
Soy Peptone (Soya Peptone)
A papaic digest of soybean meal with a high carbohydrate concentration.
It contains total nitrogen of 8.7% and NaCl of 0.4%.
Soytone
A papaic digest of soybean meal.
Special Peptone
A mixture of peptones, including meat, plant, and yeast digests. It
contains a wide variety of peptides, nucleotides, and minerals. It
contains total nitrogen of 11.7% and NaCl of 3.5%.
Thiotone™ E Peptone
An enzymatic digest of animal tissue.
Trypticase™ Peptone
A pancreatic digest of casein. It has a very low carbohydrate
content and a relatively high tryptophan content.
Tryptone
A pancreatic digest of casein. It contains total nitrogen of
12.7% and NaCl of 0.4%.
Tryptone T
A pancreatic digest of casein with lower levels of calcium,
magnesium, and iron than tryptone. It contains total nitrogen
of 11.7% and NaCl of 4.9%.
Tryptose
An enzymatic hydrolysate containing high molecular weight peptides.
It contains total nitrogen of 12.2% and NaCl of 5.7%.
Reference; http://books.google.com.au/books/about/Handbook_of_Microbiological_Media_Third.html?id=aWsF-e8tPzIC&redir_esc=y
For Synechococcus sp. PCC 7002, some protocols recommend using only the BactoAgar from Becton Dickinson (BD) for optimal growth compared to others: http://www.ncbi.nlm.nih.gov/pubmed/20960136
Thus, although the tryptone contents may be the same regardless of supplier, for optimal performance, some microorganisms may prefer Bacto-Tryptone as well...
All these informations are very important from all dears and answers .
So I thank you all.
take my respect .
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