Is there a cell penetrating peptide that I can order/synthesis that can bind non-covalently to a protein cargo, and allow it to enter a cell (Hela)?

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Zifan Gong

Hi Ewan,

Great idea to use CPP. I thought about that before I graduated. My guess is you can get some CPP synthesized with azide/alkyne group and have your cargo protein have alkyne/azide lable. Click chemistry can allow mild conjugation. But it seems to be a covalent conjugation.

You can also consider disulfide bond conjugation. After uptake, the reducing environment can break the conjugation and release the cargo.

Hope it helps.

Ewan F Dunn

Thank you Zifan.