I ran 1 ul of sybr safe and noticed that it moves to the cathode.
I thought the attraction between DNA and sybr safe is resonance bond, is that positively charged?
According the the FAQ page for SYBR Safe, the dye migrates like EtBr in a gel (towards the negative pole). You can always post-stain your gels by soaking in a buffer with SYBR safe added. Or just add in some extra SYBR to the positive end of the gel tank so the entire gel is stained evenly.
Hi Tayebe Fallahi Pashaki
as ethidium bromide, SYBR will link to nucleic acids and migrate together to allow their vizualisation but free SYBR will migrate to the neg pole (it is mainly seen as a migration front that will met the nucleic acids bands migration when all gel is stained).
fred
Just have a look at the structure:
https://en.wikipedia.org/wiki/SYBR_Green_I
Jochen Wilhelm , Frederic Lepretre , Katie A Burnette thank you so much for your valuable information,
But i ran 2ul plasmid DNA with 0.2 ul sybr safe and 0.3 ul Loading dye in the second well from the right ( i use to apply sybr safe directly into the agarose gel but this time applied it directly into the tube and then to the well) it moved toward the negative pole and plasmid DNA didn't appear in gel doc.
free, SyBr green will always run to the neg pole, until it mets some NA in the gel.
SyBr must therefore be in the gel, not in the samples.
fred
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