Somatic mutations - how to validate NGS data?

Hi, I'm searching for methods to validate somatic mutations in pancreatic beta cells. The mutations are found by Next Generation Sequencing and I have try Sanger Sequencing with no results.

12 March 2018 1,821 5 View

Glucose stimulation of rat beta cells for insulin measurement?

Can anyone suggest a protocol for glucose stimulation of rat beta cells for insulin measurement. I can see final glucose concentration as 16,7mM but I dont know why?

26 February 2018 4,742 3 View

Best way to inactivate RNase III-A?

I require 100 uL of bovine ribonuclease III-A stock solution (5mg/mL). The product from Sigma-Aldrich is a very fluffy substance. I need to inactivate this twice at 100 degree C twice. Does anyone...

31 January 2017 1,632 3 View

Normalization of small RNA-seq with housekeeping RNA?

I am working on some small RNA-Seq data and trying to normalize them. From literature, I found that people are using some housekeeping small RNA as reference for the normalization in qPCR. Can...

01 November 2016 4,047 3 View

What is production mechanism of Haft Kel field?

how is Haft Kel Field produced and how is the CO2 as injection considered in the flow simulation?

09 July 2016 882 2 View

Shelf life of TRIS?

I just started in a new lab and am about to start running western blot. However, the TRIS powder they have here is from 2002 and I'm a bit reluctant using it. Does anyone have experience using old...

26 April 2016 6,451 5 View

Does anyone know of an efficient, low-toxic caspase-1 inhibitor for in vitro cell culture?

I would like to inhibit the inflammasome in my cultured dendritic cells, but am a bit unsure of which one to buy. Does anyone have any recommendations? Thanks!

18 August 2013 2,847 2 View

Two questions: using fibronectin in transwell migration assays?

I've been having a lot of trouble trying to get my assays to work. I'm concerned that all I'm seeing is background migration. It's just B16BL6 melanoma cells (usually 1 x 10^5), using 10% FBS as a...

14 May 2013 5,684 15 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

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01 March 2021 3,905 5 View

How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

How to highlight novelty in your research paper?

I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...

28 February 2021 2,393 8 View