I conducted 16S rRNA amplicon sequencing of ileal mucosa from 16 C57BL/6 mice. I isolated DNA from the mucosal contents which were detached from the ileal tract, using DTT, and washed with a medium. Then, I applied DNA to Miseq. As a result, 99% of relative abundance at the genus level was dominated by Pseudomonas (70-80%) and Brevundimonas (19-29%) in all mice. Is this result correct? Are these genera included in mucosal microbiota?