Journal of Research in Humanities and Social Science. Is a real magazine?

Dear colleagues: I have received many emails from a magazine called ournal of Research in Humanities and Social Science. I have gone to the website of this journal, and I read "some" indexes but,...

13 December 2020 6,208 6 View

How can I connect a thermocouple to an oscilloscope?

I would like to measure temperature with a very high acquisition rate during a test that takes place in under 1ms. I was thinking of using a thermocouple and an oscilloscope. Which kind of signal...

26 October 2020 1,736 6 View

How efficient are HiTrap desalting columns for separation of proteins from free fluorescent labels?

I am looking for a reliable and fast method for separation of protein-fluorescent probe conjugates from the respective free fluorophores present in labeling reactions. HiTrap desalting columns...

06 September 2020 8,039 8 View

PhyloCode now a mediatic debate?

The recent official publication of PhyloCode has caught my attention, not for new arguments being presented for a system that has already been extensively discussed and, as I see it, whose...

04 July 2020 726 10 View

How to write a password automatically in a bash script?

I have a script that download multiples files from a server, but for each one it requires me to write a password. These are 1000, so it would be laborious to write 1000 times the password. How...

02 April 2020 852 2 View

How to define parametric modulators for multiple conditions in SPM?

Dear RG Community, I am performing an SPM analysis in which I've got 8 conditions plus the baseline in an event-related experiment. Each condition has 4 trials, totalling 32 trials per run. There...

02 April 2020 7,060 1 View

Alkali Resistance of SS316, Nickel and Hastelloy?

Does anyone know or may anyone assist me to find which of these materials (Stainless Steel 316, Nickel, or Hastelloy) is the most resistant to a highly concentrated NaOH solution (>27 mol/L)...

26 March 2020 6,875 6 View

Recommendations/tips on publishing datasets?

In my research about vibration of hermetic compressors, I'm creating a dataset that I intend to make it available once I publish the paper it refers to. Do you have any tips or recommendations on...

18 March 2020 7,803 5 View

Tools for ncRNA in bacteria?

Hello, I am trying to determine if a region present in a Streptomyces genome acts as a ncRNA that affects gene expression. Which are the best tools to analyze sequences for this? Thanks!

24 November 2019 5,270 3 View

In Limma package, why should I linearize my data in some analyses?

tl;dr: Why data linearization is applied in the identification o differentially expressed genes/proteins? I am new to data analysis of big data like proteomics. As far as I know, a simple t-test...

16 November 2019 8,441 4 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

Do you think it is feasible to have a 100% Renewable Energy-based Electricity System in Bangladesh in the distant future?

In Bangladesh, the proved reserved of natural gas 34 TCF which will lead the country next 20 years and currently 82% natural gas consumed in the power sector for power production. Whereas only 3%...

01 February 2021 251 12 View

How to add journals not listed on Research Gate ?

I have two publications one listed in the web of science master list " European Online Journal of Natural and Social Sciences " ISSN: 1805-3602 and Second " Journal of Managerial Sciences"...

26 January 2021 7,158 7 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View

Good day, I have not been able to amplify the plasmid Frt-hspB1. Somebody could give an advice me?

I acquired the plasmid in Addgene, reconstituted the filter paper where the plasmid comes from with 30 microliters of TE buffer pH 8, immediately afterwards perform the transformation of E.coli...

25 January 2021 2,526 3 View

Can I use MERRA-2 model's "500hpa geopotential height" monthly data in excel form for districts or I download the data in netcdf files and extract it?

I want to use MERRA-2 model's monthly data of 500hpa geopotential height for different districts of Pakistan (1980-2019). I want to download this data from NASA Giovanni and I want to select time...

25 January 2021 6,144 1 View