Sorry but the question is very much unclear to me. What do you mean by subtract the column? Do you want to remove it or perform the arithmetic operation of subtraction among the mentioned columns. I will edit this answer accordingly.

Yes, I want to perform the arithmetic operation of subtraction among the mentioned columns, thanks for helping.

1 1 ===>> 0

2 2 0

3 3 0

4 4 0

5 5 0

You could create a bash (shell) script with 'awk' commands to do this. Ideally, extract each column from file and put into temp files, then use 'paste' to combine temp files, then final 'awk' command to perform subtraction, you can also include commands to clean up temp files etc within the shell script.

You can do the substraction and write the result just using awk from command line.

just try:

awk '{$3 = $1 - $2} 1' < your_input_file > your_output_file

you might formating field separator of the output (if you need) .