Is it possible to stain heme in cell culture?

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Adam B Shapiro

Can you use the visible light absorbance (red color) of heme in an extract of the culture to measure it's amount?

Benjaporn Kiatpakdee

Hello Adam,

Thank you for your advice but I just want to see the location of heme within the cell if it's possible.

You might be able to use the fluorescence technique described here:

https://www.ncbi.nlm.nih.gov/pubmed/4285675

Thank you for your kind advice again. Unfortunately, I don't have that kind of microscope in my lab : (