How to determine a primer sequence with ambigous bases?

I would like to know how we can assign a nucleotide of a primer with ambiguity codes. Take this primer which targets the nosZ gene in bacteria for example; 5'-CGCRACGGCAASAAGGTSMSSGT-3' (Henry...

02 March 2018 470 3 View

(Picture is attached) What can be done to produce a DGGE gel with good quality?

04 May 2016 9,860 7 View

What is the recommended amount of PCR product to load on DGGE?

I performed PCR which targets bacterial 16S (using GC968f/1401r) and fungal 18S (using NS1/GCFung), each produced ~400 bp and ~350 bp, receptively. The DNA was extracted from soil sample.Can...

03 April 2016 7,212 4 View

No title

My soil sample is of sandy loam. I've done the extraction protocol several times, precisely as stated in the manufacturer's instruction manual but I did not get any good result. The concentration...

02 March 2016 3,899 3 View

What is the significance of microbial biomass for carbon and nitrogen mineralization?

Microbial biomass changes rapidly in response to changes in soil properties, but how to interpret the increase or decrease of microbial biomass carbon and microbial biomass nitrogen in relation to...

31 December 2015 6,221 2 View

Discrete Phase model (DPM) particle break-up has particle size limits during particle tracking?

Hello, I am currently working on simulating water droplet partciles in a closed room. When I use uniform particles, without "particle break-up", I can carry out the simulation without any problem....

02 March 2021 3,053 2 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Zno nano particles colour changed from white to grey in process of calcination any suggestions?

In the formation of Zno nano particles process, after drying at 120 degree C, my zno nano particles are completely white but when i calcine it for 450 degree C it converts into grey colour. i...

26 February 2021 2,435 4 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View