02 February 2018 1 4K Report

I intend to obtain biochar particles without the labile fraction. Is there any available method to achieve that?

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How to determine a primer sequence with ambigous bases?

I would like to know how we can assign a nucleotide of a primer with ambiguity codes. Take this primer which targets the nosZ gene in bacteria for example; 5'-CGCRACGGCAASAAGGTSMSSGT-3' (Henry...

02 March 2018 582 3 View

(Picture is attached) What can be done to produce a DGGE gel with good quality?

DGGE was carried out on 7.5 % polyacrylamide gel  in 1× TAE buffer with a denaturing gradient from 30% to 60% (at 60 °C; 70 V; 16 h). Each lane were loaded with 700 ng of DNA (PCR amplicon of...

04 May 2016 9,931 7 View

What is the recommended amount of PCR product to load on DGGE?

I performed PCR which targets bacterial 16S (using GC968f/1401r) and fungal 18S (using NS1/GCFung), each produced ~400 bp and ~350 bp, receptively. The DNA was extracted from soil sample.Can...

03 April 2016 7,283 4 View

I used PowerSoil® DNA Extraction Kit (MOBIO) to extract DNA directly from soil but it was unsuccessful. Which step of the protocol can be modified?

My soil sample is of sandy loam. I've done the extraction protocol several times, precisely as stated in the manufacturer's instruction manual but I did not get any good result. The concentration...

02 March 2016 3,978 3 View

What is the significance of microbial biomass for carbon and nitrogen mineralization?

Microbial biomass changes rapidly in response to changes in soil properties, but how to interpret the increase or decrease of microbial biomass carbon and microbial biomass nitrogen in relation to...

31 December 2015 6,295 2 View

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