Is it possible to measure clorophyll fluorescence in the night? Just to avoid the wasteful process of shading each leaf with a little piece of black cardboard during 30 minutes. Thank you.
If you are interested only in parameters at dark adapted state (e.g. Fv/Fm), yes, it is possible. If you need also parameters at light adapted state, you should illuminate the plants and wait at least 30 min.
in the night there should be real darkness but handling instrumentation requires some light. the fluencce for activating LHC is very low. The use of leaf shutter clips and recording F0 relaxation with dark adaptation lenght are useful info. http://pcp.oxfordjournals.org/content/50/9/1600.full.pdf
You can use a light tool and standardize with the real sunlight using light meter for error correction. Alternatively, you can also use SPAD or another tool likely.