I read a paper that said it wasn't really feasible, has anyone had any success?
The question is what would any change in salivary BDNF measure. Blood levels are bad o
By blood levels are bad do you mean that the Elisas are unreliable? This would just be a cross sectional study so no changes in BDNF, but ideally the salivary measure would be a means of evaluating circulating levels, just not sure if it's accurate.
There would little reason for BDNF to be expressed in saliva. Are you sure you are not confusing it with corticosterone?
BDNF can be seen in saliva, in fact in animal models some have reported that the salivary glands represent the source of circulating plasma levels. Most of the studies I've looked at are relatively small however, and I'd love confirmation that salivary levels are directly correlated with serum levels.
Please, we tryied to measure by ELISA salivary BDNF. We used kit by Promega, Cloude clone, Biosensis (ng/ml) but without any good results. Have you any suggetsions? Thanks
These folks created an effective salivary ELISA, but it doesn't measure circulating BDNF levels, just what's released by the salivary glands. Not sure what your measures are, or whether that'd be useful to you. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3046426/
One way to measure BDNF more easily is to just genotype for the Val/Met polymorphism, but that obviously doesn't get you specific person to person levels.
The question is, why wouild anybody interested in brain BDNF want to measure salivay gland BDNF.
Dear Cooper,
My answer is "NO".
Some papers report measurable salivary BDNF levels (e.g. Mandel et al. 2011):
Article Brain-derived Neurotrophic Factor in Human Saliva: ELISA Opt...
However, the recent paper by Vrijen et al. (2017):
Article Measuring BDNF in saliva using commercial ELISA: Results fro...
concludes that BDNF cannot be measured reliably in saliva. This is because the amount of BDNF in saliva is below the limit of detection (LOD) of currently available ELISAs.
I tried to replicate BDNF quantification in salivary in my lab and also failed to reach the concentrations higher than LOD. I used "Total BDNF" ELISA assay (R&D), which is not validated to salivary protein.
Even if you will find a sensitive enough method of measurement, please take into consideration the following possible pitfalls:
1) BDNF may be detected as proBDNF glycosilated form or mature BDNF molecule - which appear to elicit distinct, and often opposite, effects in the mouth.
2) sampling method may be important as passive expectoration usually recover more BDNF than Salivette.
3) BDNF may be degraded by proteases, soit would be benefitial to add protease inhibitors to the sample right affter collection of the material.
Michal
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