Hello,
I am planning to conduct an experiment to identify bacteria bound to IgG by flow cytometry. I aim to focus on live bacteria, so I intend to use the LIVE/DEAD BacLight Kit (Syto9 and propidium iodide) to confirm I'm examining live bacteria. Since I need to fix the samples before acquisition on the flow cytometer, my questions are:
Thanks in advance,
Yes, it is possible to fix bacteria after performing LIVE/DEAD staining using the BacLight Kit. LIVE/DEAD staining is a commonly used method to differentiate live bacteria from dead bacteria based on the integrity of their cell membranes. The staining kit typically contains two fluorescent dyes: SYTO 9, which stains both live and dead bacteria with intact membranes, and propidium iodide (PI), which only penetrates bacteria with compromised membranes and stains them red.
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