I have a protein cloned in either pGEX-6P1 or pQE30 vector. It is not known to me. I am trying to induce the vector in XL1blue cells. how would I be able to verify which vector is it without sequencing.
Two ways: one is to extract the plasmid with miniprep and have a expected size of restriction pattern in the backbone, or to have the standard primers to amplify insert near multiple cloning site, test if the PCR product is obtained and also subject it to restriction in case you know the sequence of the CDS
With this little (practically none) information you should not proceed for it may result in waste of time and resources. There are lots of factors that need to be examined carefully; copy number, promoter, selection marker etc. Please try to obtain more information about the vector first.
Thank you guys, the vector seems to be pGEX 6PA1. I have cut the vector with bamH1. The DNA gel showed the cut vector aligned with the molecular weight that was expected (vector + insert).
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