Hello,
I want to know if STH and schistosome eggs are resistant to freezing and can be detected a second time if forzen feces are reanalyzed by the Ritchie concentration method and Kato-Katz.
Thanks.
In agreement with Hala Elwakil and Hairul Hafiz Mahsol, eggs of STH and Schistosomes could be subjected to mechanical and molecular distortion as a result of the extremely low temperature associated with freezing. Hence, they could only be useful for prevalence/ intensity studies.
We found eggs of Hymenolepis nana in frozen feces, but the eggs of schistosomes have a much thinner shell, so it's likely that when frozen, it collapses
STH and schistosoma eggs can be found in frozen stool sample but you may found them distorted
Yes, I am agreed with Ermakova and Elwakil. You may recognised the eggs as STH and schistosmes only for prevalence discovery or incidence study, not for identification species or taxonomy.
Good luck!
In agreement with Hala Elwakil and Hairul Hafiz Mahsol, eggs of STH and Schistosomes could be subjected to mechanical and molecular distortion as a result of the extremely low temperature associated with freezing. Hence, they could only be useful for prevalence/ intensity studies.
I agree with Mr. Atalabi Tolulope n Mr.Hairul Hafiz. STH and Schistosomes examination using frozen stool can be done as a practice way to determine prevalence study.
I am in agreement with Hala Elwakil Mahsol Hairul and Hafiz, eggs of schistosomes and STH could be submitted to a distortion molecular mechanics. Very low temperatures quickly destroy the eggs of hookworm duodenal. By against conservation at 4°C and 8°C during 24h would make a diagnosis parasitologist molecular and without worry and even make studies of prevalence/intensity.
It depends on the refrigeration temperature of the sample and the number of days.If the samples are refrigerated at +4 during a day, you can make the identification of the species the following day.for more than two days, I think Not that it is possible to identify
Hello!! I am reanalyzing the forzen samples of feces and I am finding many samples with Trichuris trichuria and Ascaris lumbircoides eggs. However, in several samples I could observe stranges bodys that I can not identify. I dont know if they are polen grains, artafacts or destroied eggs. The size is aproximatly as an ascaris egg. Can anyone help? Thanks
yes it is possible to find STH and schistosoma eggs in frozen stool sample but you may found them distorted .
It is possible to to diagnose STH and schistosome infections by microscopy in frozen samples of feces but they may appear distorted.
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