The existence of negative pressure of vacuum follows from the cosmological models, based on the results of observations. The gravitational defect of mass is interpreted as the transfer of energy to the vacuum, which becomes apparent from its deformation.The gravitational impact of matter on the vacuum and opposite in the sign pressure of it can be determined in case of weakly gravitating static centrally symmetric distribution of matter. A possibility to extend the obtained results to arbitrary gravitational systems is evaluated. The equation of state (p_v =-(1/3)[q_L+q_m+q_rel]) gives the deceleration parameter of the universe consistent with its accelerating expansion.