Is it possible to decrease the amount of sulfuric acid to be added (according to literature is 10 mL) to quantify PHB concentration?

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Does the GC pre-vacuum pump have an optimum operating temperature?

In the lab, the temperature is often around 30 degrees Celsius and lately, the pre-vacuum pump has often stopped working. I wondered if this high temperature could cause the constant (daily)...

05 September 2022 556 4 View

Is there any other ammonia-oxidizers bacteria beside Nitrosomonas which are found in high level in soils?

I'm going to do an experiment in lab in which I examine the effect of nitrapyrin ( it is a nitrification inhibitor ) on more ammonia-oxidizers bacteria. There are several experiments proved that...

11 February 2019 3,913 6 View

How can I analyse alcohols (methanol, ethanol, acetone, 2-propanol) in water solution with GC-FID?

I would like to analyse alcohols in water solution with GC-FID. I have MEGA-624 column: 0.32 mm x 1.80 µm x 60 m.But the stationary phase consist siloxane, so water will damage this phase. I use...

08 October 2016 7,186 20 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

My cfDNA extraction protocol using silica beads works with plasma collected in COD SAGM bags but not with plasma collected in K2 EDTA tubes. Why so?

I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...

28 July 2024 3,559 0 View

What is the Principle of Cysteine-carbazole-sulfuric acid reaction with glucose?

I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...

23 July 2024 3,891 0 View

Why methanol and sulphuric acid used in the analysis of polyhydroxyalkanoates (PHA) by GC-MS?

Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?

22 July 2024 1,210 2 View

Can sulfuric acid be used to prepare anthrone reagent when it has oxidized and changed color in reaction to light?

Sulfuric acid has changed color to yellow.

16 July 2024 6,964 0 View

Qiagen circulating nucleic acid kit poor yield?

Hi, Attempted to extract cfDNA from a 4 mL plasma sample, with poor yield. Went through the manual in the section regarding poor yield, and feel that this does not apply. Some potential...

14 July 2024 7,475 1 View

How to prepare materials before protein-peptide or protein-protein docking?

Hi all, I am conducting research on screening peptides (20-35 amino acids) with potential for treating dengue fever through protein-peptide docking methods. In the protein database, I found the...

10 July 2024 8,037 1 View