Is it possible to decrease the amount of sulfuric acid to be added (according to literature is 10 mL) to quantify PHB concentration?

I have been quantifying PHA produced by bacteria via crotonic acid assay. According to literature it is necessay to add 10 ml of sulfuric acid to the PHB sample to react at 95°C during 20 min, and...

10 October 2017 2,978 2 View