How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

Amorphous silica XRD peak at around 10 degree?

21 February 2021 3,417 5 View

How could I get the P-value from the mean, SEM, and n of different groups in the t-test?

How could I get the P-value from the mean, SEM, and n of different groups in the t-test without the original data? For detail, I would like to know the P-value between BMP-2: 50ng/ml group and the...

06 February 2021 6,539 4 View

What mechanism is involved when chitosan is coated on magnetic nanoparticles?

Hello! I am currently working on coating of chitosan on magnetic Fe3O4 nanoparticles. I am wondering how this coating process happened? i.e. through what mechanism that chitosan can be coated on...

13 January 2021 5,657 5 View

The impact of different poisson's ratios when analysising field vibration?

The Poisson's ratio of soil is quite difficult for us geo-engineers to obtain. I came across a problem when I tried to determine the parameters in a FEM model. For the saturated clay in...

02 January 2021 8,971 3 View

Is it possible to deposition a large area dielectric layer(mm scale) on the wet transferred graphene?

I want to deposition a dielectric layer on top of the CVD wet transferred graphene. The purpose of the dielectric layer is to uniformly cover the graphene and enable the top-gate modification of...

14 December 2020 7,693 2 View

Is the fluorescent protein must be totally photobleached in FRAP assay?

Hi I have tried to measure the mobility of a protein of interest by the FRAP assay. The protein was fused with GFP. I overexpressed the fusion protein in Hela cells. However, when the area of the...

14 December 2020 6,567 1 View

Is there some effective transfection reagents or methods for transfecting plasmids to ExpiCHO-S cells?

Dear all, We want to transfect pCDH plasmid or other plasmids to suspension ExpiCHO-S cells and express our target proteins, and we used the transfection reagents PEI. Unfortunately, there...

26 November 2020 8,323 3 View

No title

The cell I used is 293T.I want to it can grow on the biocompatible materials,like agarose or alginate gels.But the shape of the cells is not good.In the T25 flask you will find the cell like...

26 November 2020 1,009 2 View

Again, what are the bands of extracted plasmid?

I used Trans1-T1 phage resistant chemically competent cells to extracted lentiviral gRNA expression vectors. And there were bands near 2000bp and smear on 1% argrose gel. Did the smear come from...

25 November 2020 858 2 View

The most suitable method to calculate the stress intensity factor in the presence of contact between the crack lips?

I'm trying to study cracked structures under a compressive load taking into account contact between the crack lips. I would like to know if the method of extrapolation by displacement remains...

21 February 2021 8,694 4 View

What is the future of biogas in Asia?

What is the future of biogas in Asia?

31 December 2020 8,821 16 View

What country in Asia produces the most renewable energy?

What country in Asia produces the most renewable energy?

25 December 2020 8,762 24 View

Please name companies in Asia or India who carry out whole human genome sequencing with high quality for less cost?

22 December 2020 4,495 3 View

Most of the plastic is shipped to Asia?

Dear Sir I came across your paper. First of all, congratulations on your amazing research article. Loved it! Also, I am very interested to know, why is the waste that is collected in the...

14 December 2020 6,342 4 View

Does anyone know of any South American or African frogs with ultrasonic components in their calls?

There are a few examples of Asian frogs (Odorrana and Huia) with advertisement calls that contain either audible and ultrasonic components, or purely ultrasonic components, I was wondering if...

26 October 2020 2,906 16 View

Is there any open datasource to study sea level change in parts of Asia?How to predict the rate of SLR for coming 50 years ahead?

I am looking for datasets to study sea level change in parts of Asia since i do not have any field observation. I searched in NASA Sea level portal but it left me confused how to process those...

09 October 2020 9,795 1 View

Is there any open datasource to study sea level change in parts of Asia?How to predict the rate of SLR for coming 50 years ahead?

I am looking for datasets to study sea level change in parts of Asia since i do not have any field observation. I searched in NASA Sea level portal but it left me confused how to process those...

08 October 2020 3,152 1 View

Literature on traditional/ancient slaughtering patterns of goats/sheep?

Any recommendations? I'm mostly (but not exlusively) interested in eastern Mediterranean and West Asia...

09 September 2020 7,344 3 View

Which is the best system to keep endocrinology samples frozen in the field, without electricity?

Hi everyone, I am looking for suggestions to store biological samples in the field for endocrinology analyses, where I will not have electricity. One of the options we are evaluating is a solar...

01 September 2020 3,766 3 View