Dear Dr. Ezatollah Mosavi

Ammonium sulfate is not used in SDS-PAGE. Instead, ammonium persulfate is used with TEMED (Tetramethylethylenediamine) as a catalyst for polymerization of acrylamide when preparing SDS-PAGE.

Alternate chemical names for ammonium persulfate includes ammonium peroxydisulfate or ammonium peroxodisulfate.

So, you can use ammonium peroxydisulfate or ammonium peroxodisulfate or ammonium persulfate in SDS-PAGE.

Regards,

Malcolm Nobre

Hi there, from what I gathered I am pretty sure that APS(Ammonium Peroxodisulfate) helps out the gel polymerization in combination with TEMED. Ammonium Sulfate is not involved in the polymerization of acrylamide! If APS is unavailable, you may use an alternative initiator like potassium peroxodisulfate.

Ammonium peroxydisulfate and ammonium persulfate are two names for the same thing. Use it for PAGE gel polymerization, not ammonium sulfate.