E.g.
The maximum absorbance of analyte 'A' coming to be @ 356.4, 356
Literature gives the idea of maximum absorbance for the same analyte 'A' @ 360 nm.
Will it be a problem to proceed with 356.4 nm or should i follow 360 nm.
Dear Manoj,
According to my knowledge absorbance for any analyte varies a bit with respect to time and type of UV-spec etc... I have observed in some research articles, they mentioned maximum absorbance in ranges instead of single wavelengths.
So you can proceed with the wavelength for which you get maximum absorbance.
Dear Manoj,
I agree with Mr.Suresh.For your analyte, the λmax obtained by your sample.λ range in literature are only guidelines.
The registered lambda max depends on how accurately the spectrometer is calibrated, yours and the one used for the literature reports. Lmax can also appear to shift if the sample is very concentrated and the spectrometer is close to saturation. There can also be solvatochromatic shifts in different solvents.
Usually, a few nanometers is acceptable.
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